MTCO3 Polyclonal antibody

MTCO3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55082-1-AP

Synonyms

COIII, COXIII, cytochrome c oxidase III, Cytochrome c oxidase subunit 3, MT CO3, MTCO3



Tested Applications

Positive WB detected inmouse kidney tissue, C2C12 cells, mouse liver tissue, rat kidney tissue, rat liver tissue
Positive IHC detected inmouse cerebellum tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55082-1-AP targets MTCO3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cytochrome c oxidase III
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 32-35 kDa
GenBank Accession NumberYP_003024032
Gene Symbol COX3
Gene ID (NCBI) 4514
RRIDAB_2881265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTCO3 (cytochrome c oxidase subunit III) is a mitochondrial DNA encoded subunit of respiratory Complex IV which is located within the mitochondrial inner membrane and is the final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. The predicted MW of MTCO3 is 30 kDa, while it had been observed as 23 kDa in some papers (PMID: 17557076).

Protocols

Product Specific Protocols
WB protocol for MTCO3 antibody 55082-1-APDownload protocol
IHC protocol for MTCO3 antibody 55082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Res

Active RNA interference in mitochondria.

Authors - Kuanxing Gao
mouseWB

Diabetes

Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis.

Authors - Na Tian
mouseWB

Int J Mol Sci

Mitochondrial rRNA Methylation by Mettl15 Contributes to the Exercise and Learning Capability in Mice.

Authors - Olga A Averina
humanWB

J Med Genet

COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency.

Authors - Anabel Martinez Lyons
mouseIF

Sci China Life Sci

Cholesterol homeostasis regulated by ABCA1 is critical for retinal ganglion cell survival.

Authors - Jialiang Yang
humanWB

Carcinogenesis

Mitochondrial miR-181a-5p promotes glucose metabolism reprogramming in liver cancer by regulating the electron transport chain.

Authors - Xiang Zhuang