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AEG-1/MTDH-Specific Polyclonal antibody
AEG-1/MTDH-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 13860-1-AP
|Positive WB detected in||MCF-7 cells, PC-3 cells, A375 cells, HepG2 cells, HeLa cells, RAW 264.7 cells, mouse spleen tissue, DU 145 cells, A549 cells, HL-60 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, mouse brain tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13860-1-AP targets AEG-1/MTDH-Specific in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AEG-1/MTDH-Specific fusion protein Ag4840|
|Calculated molecular weight||582 aa, 64 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC045642|
|Gene ID (NCBI)||92140|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH.
|Product Specific Protocols|
|WB protocol for AEG-1/MTDH-Specific antibody 13860-1-AP||Download protocol|
|IHC protocol for AEG-1/MTDH-Specific antibody 13860-1-AP||Download protocol|
|IF protocol for AEG-1/MTDH-Specific antibody 13860-1-AP||Download protocol|
|IP protocol for AEG-1/MTDH-Specific antibody 13860-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Cell Mol Med
CCL18 promotes the metastasis of squamous cell carcinoma of the head and neck through MTDH-NF-κB signalling pathway.
Hypoxia promotes migration/invasion and glycolysis in head and neck squamous cell carcinoma via an HIF-1α-MTDH loop.
J Mol Neurosci
Upregulation of AEG-1 Involves in Schwann Cell Proliferation and Migration After Sciatic Nerve Crush.
Metadherin Regulation of Vascular Endothelial Growth Factor Expression Is Dependent Upon the PI3K/Akt Pathway in Squamous Cell Carcinoma of the Head and Neck.
BMC Complement Med Ther
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
eman (Verified Customer) (11-26-2020)
this antibody works very well incubating either at room temperature or overnight 2 dilutions are tested 1:1000 and 1:2000 IF not tested yet