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  • KD/KO Validated

AEG-1/MTDH-Specific Polyclonal antibody

AEG-1/MTDH-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13860-1-AP

Synonyms

3D3, 3D3/LYRIC, AEG 1, AEG1, LYRIC, LYRIC/3D3, metadherin, Metastasis adhesion protein, MTDH, Protein LYRIC



Tested Applications

Positive WB detected inMCF-7 cells, PC-3 cells, A375 cells, HepG2 cells, HeLa cells, RAW 264.7 cells, mouse spleen tissue, DU 145 cells, A549 cells, HL-60 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13860-1-AP targets AEG-1/MTDH-Specific in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AEG-1/MTDH-Specific fusion protein Ag4840
Full Name metadherin
Calculated molecular weight 582 aa, 64 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC045642
Gene symbol MTDH
Gene ID (NCBI) 92140
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH.

Protocols

Product Specific Protocols
WB protocol for AEG-1/MTDH-Specific antibody 13860-1-APDownload protocol
IHC protocol for AEG-1/MTDH-Specific antibody 13860-1-APDownload protocol
IF protocol for AEG-1/MTDH-Specific antibody 13860-1-APDownload protocol
IP protocol for AEG-1/MTDH-Specific antibody 13860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Cell Mol Med

CCL18 promotes the metastasis of squamous cell carcinoma of the head and neck through MTDH-NF-κB signalling pathway.

Authors - Yuexiang Qin
  • KD Validated

Oncol Rep

Hypoxia promotes migration/invasion and glycolysis in head and neck squamous cell carcinoma via an HIF-1α-MTDH loop.

Authors - Gangcai Zhu
ratWB,IHC,IF

J Mol Neurosci

Upregulation of AEG-1 Involves in Schwann Cell Proliferation and Migration After Sciatic Nerve Crush.

Authors - Youhua Wang
  • KD Validated
humanWB,IHC

N/A

N/A

Authors - N/A
humanWB, IHC

Medicine (Baltimore)

Metadherin Regulation of Vascular Endothelial Growth Factor Expression Is Dependent Upon the PI3K/Akt Pathway in Squamous Cell Carcinoma of the Head and Neck.

Authors - Gang-Cai Zhu
humanWB,IF,CoIP

BMC Complement Med Ther

PMID: 32066429

Authors - Li Han