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MTM1 Polyclonal antibody

MTM1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13924-1-AP

Synonyms

CG2, CNM, MTM1, MTMX, Myotubularin, myotubularin 1, XLMTM

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using MTM1 Polyclonal antibody (13924-1-AP)

    WB analysis of L02 using 13924-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 13924-1-AP (MTM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MTM1 Polyclonal antibody (13924-1-AP)

    WB analysis of Jurkat using 13924-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13924-1-AP (MTM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using MTM1 Polyclonal antibody (13924-1-AP)

    IHC staining of rat skeletal muscle using 13924-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 13924-1-AP (MTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using MTM1 Polyclonal antibody (13924-1-AP)

    IHC staining of rat skeletal muscle using 13924-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 13924-1-AP (MTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MTM1 Polyclonal antibody (13924-1-AP)

    IHC staining of mouse skeletal muscle using 13924-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13924-1-AP (MTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MTM1 Polyclonal antibody (13924-1-AP)

    IHC staining of mouse skeletal muscle using 13924-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13924-1-AP (MTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using MTM1 Polyclonal antibody (13924-1-AP)

    IF Staining of L02 using 13924-1-AP

    Immunofluorescent analysis of L02 cells using 13924-1-AP (MTM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inL02 cells, Jurkat cells
Positive IHC detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13924-1-AP targets MTM1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTM1 fusion protein Ag4940
Full Name myotubularin 1
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC030779
Gene Symbol MTM1
Gene ID (NCBI) 4534
RRIDAB_2147700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MTM1(Myotubularin) gene encodes a protein that belongs to a family of putative tyrosine phosphatases. It is required for muscle cell differentiation. The protein is also a potent phosphatidylinositol 3-phosphate (PI3P) phosphatase, which is a 66 kDa ubiquitous protein that is found mutated in the X-linked genetic disorder myotubular myopathy(XLMTM).MTM1 could therefore act as a regulator of the highly active phosphoinositide (PI) metabolism in cells, where interconversions of the eight PIs described so far is controlled by a set of specific kinases and phosphatases(PMID:14660569). MTM1 has two isoforms with the molecular mass of 66kDa and 70 kDa.

Protocols

Product Specific Protocols
WB protocol for MTM1 antibody 13924-1-APDownload protocol
IHC protocol for MTM1 antibody 13924-1-APDownload protocol
IF protocol for MTM1 antibody 13924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Siting (Verified Customer) (01-28-2020)

This antibody worked for western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue