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  • Featured Product
  • KD/KO Validated

MUS81 Polyclonal antibody

MUS81 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11018-1-AP

Synonyms

MUS81

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MUS81 Polyclonal antibody (11018-1-AP)

    WB analysis using 11018-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11018-1-AP (MUS81 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using MUS81 Polyclonal antibody (11018-1-AP)

    WB analysis of NIH/3T3 using 11018-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11018-1-AP (MUS81 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MUS81 Polyclonal antibody (11018-1-AP)

    IF Staining of MCF-7 using 11018-1-AP

    Immunofluorescent analysis of MCF-7 cells, using MUS81 antibody 11018-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHepG2 cells, NIH/3T3 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11018-1-AP targets MUS81 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUS81 fusion protein Ag1476
Full Name MUS81 endonuclease homolog (S. cerevisiae)
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 52-72 kDa
GenBank Accession NumberBC009999
Gene Symbol MUS81
Gene ID (NCBI) 80198
RRIDAB_2147130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Crossover junction endonuclease MUS81 has associated endonuclease activity against structure-specific oligonucleotide substrates, including synthetic Holliday junctions(PMID:11741546). It interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The open reading frame of human MUS81 predicts a translation product of 551 amino acids with a molecular mass of 59 kD. There are some reports showed that the enzyme can express a 72 kDa protein in human tissues(PMID:12724407).

Protocols

Product Specific Protocols
WB protocol for MUS81 antibody 11018-1-APDownload protocol
IF protocol for MUS81 antibody 11018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell

EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion.

Authors - Antonio Marzio
  • KD Validated
View Article
WB

Elife

53BP1 and BRCA1 control pathway choice for stalled replication restart.

Authors - Yixi Xu
View Article