MYH3 Polyclonal antibody

MYH3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22287-1-AP

Synonyms

eMyHC, HEMHC, MYH3, MYHC EMB, MYHSE1, Myosin 3, Myosin heavy chain 3, SMHCE



Tested Applications

Positive WB detected inmouse embryo tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inrat heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22287-1-AP targets MYH3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myosin, heavy chain 3, skeletal muscle, embryonic
Calculated Molecular Weight 234 kDa
Observed Molecular Weight 220-230 kDa
GenBank Accession NumberNM_002470
Gene Symbol MYH3
Gene ID (NCBI) 4621
RRIDAB_2879060
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MYH3 antibody 22287-1-APDownload protocol
IHC protocol for MYH3 antibody 22287-1-APDownload protocol
IP protocol for MYH3 antibody 22287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

A non-canonical tricarboxylic acid cycle underlies cellular identity.

Authors - Paige K Arnold
ratWB

Basic Res Cardiol

Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.

Authors - Alzbeta Hulikova
mouseWB

Int J Mol Sci

Molecular and Metabolic Mechanism of Low-Intensity Pulsed Ultrasound Improving Muscle Atrophy in Hindlimb Unloading Rats.

Authors - Lijun Sun
mouseWB,IF

FASEB J

miR-206 family is important for mitochondrial and muscle function, but not essential for myogenesis in vitro.

Authors - Roza K Przanowska

Am J Pathol

B4GALNT2 (GALGT2) Gene Therapy Reduces Skeletal Muscle Pathology in the FKRP P448L Mouse Model of Limb Girdle Muscular Dystrophy 2I.

Authors - Paul J Thomas
bovineWB,IF

Front Physiol

Podocan Promotes Differentiation of Bovine Skeletal Muscle Satellite Cells by Regulating the Wnt4-β-Catenin Signaling Pathway.

Authors - Shuang Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alessandra (Verified Customer) (03-23-2022)

Antigen retrieval was performed on Tibialis Anterior muscle cross sections. MYH3 in red (newly formed fibers); IgG in cyan (dying muscle fibers)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: Skeletal Muscle/Myoblast
MYH3 Antibody Immunofluorescence validation (1:300 dilution) in Skeletal Muscle/Myoblast (Cat no:22287-1-AP)