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MYL3 Monoclonal antibody

MYL3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E4B2

Cat no : 66286-1-Ig

Synonyms

Cardiac myosin light chain 1, CMH8, CMLC1, MLC1SB, MLC1V, MYL3, Myosin light chain 3, VLC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYL3 Monoclonal antibody (66286-1-Ig)

    WB analysis using 66286-1-Ig

    Various tissue lysates were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using MYL3 Monoclonal antibody (66286-1-Ig)

    WB analysis of human skeletal muscle using 66286-1-Ig

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using MYL3 Monoclonal antibody (66286-1-Ig)

    WB analysis of mouse heart using 66286-1-Ig

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using MYL3 Monoclonal antibody (66286-1-Ig)

    IHC staining of human heart using 66286-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MYL3 Monoclonal antibody (66286-1-Ig)

    IHC staining of human heart using 66286-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYL3 Monoclonal antibody (66286-1-Ig)

    IHC staining of human skeletal muscle using 66286-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYL3 Monoclonal antibody (66286-1-Ig)

    IHC staining of human skeletal muscle using 66286-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens).

Tested Applications

Positive WB detected inhuman skeletal muscle tissue, mouse heart tissue, human heart tissue, pig heart tissue, rat heart tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66286-1-Ig targets MYL3 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Reactivity human, rat, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYL3 fusion protein Ag24535
Full Name myosin, light chain 3, alkali; ventricular, skeletal, slow
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC009790
Gene Symbol MYL3
Gene ID (NCBI) 4634
RRIDAB_2881669
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)

Protocols

Product Specific Protocols
WB protocol for MYL3 antibody 66286-1-IgDownload protocol
IHC protocol for MYL3 antibody 66286-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols