Myocilin Polyclonal antibody

Myocilin Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14238-1-AP

Synonyms

GLC1A, GPOA, JOAG, JOAG1, MYOC, Myocilin, TIGR



Tested Applications

Positive WB detected inhuman heart tissue, human stomach tissue, mouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

14238-1-AP targets Myocilin in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Myocilin fusion protein Ag5492
Full Name myocilin, trabecular meshwork inducible glucocorticoid response
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC029261
Gene Symbol MYOC
Gene ID (NCBI) 4653
RRIDAB_2148652
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622). MYOC has a molecular weight of 55-57 kDa, besides, a 66-kDa form, which could be a result of post-translational modifications, has also been reported in some researches (PMID: 9497363; 12150989).

Protocols

Product Specific Protocols
WB protocol for Myocilin antibody 14238-1-APDownload protocol
IP protocol for Myocilin antibody 14238-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Ann Transl Med

Adenosine A3 receptor activated in H2O2 oxidative stress of primary open-angle glaucoma.

Authors - Ziyu Zhou
humanWB

Int J Ophthalmol

Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork

Authors - Miao Wei
mouseWB

J Ocul Pharmacol Ther

Activation of Sonic Hedgehog Signaling Pathway Regulates Human Trabecular Meshwork Cell Function

Authors - Xiaochen Wang