Mouse CCL22 ELISA Kit

Sensitivity

0.98 pg/mL

Range

7.8-500 pg/mL

Reactivity

Mouse

Cat no : KE10053

Synonyms

Ccl22, DCBCK, MDC, Scya22



Product Information

KE10053 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL22 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL22. The assay recognizes mouse CCL22. An antibody of biotinylated specific for CCL22 has been pre-coated onto the microwells. The CCL22 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CCL22 is added to detect the captured mouse CCL22 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse CCL22 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.98 pg/mL
Range 7.8-500 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 20299

Recovery

Sample TypeAverageRange
Mouse serum103%85%-121%
Cell culture supernatants101%83%-124%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120251.26.72.7
22056.32.54.5
32012.21.29.5

InterAssay

Samplenmean ( pg/mL)SDCV%
124215.920.99.7
22455.73.46.0
32412.71.39.9

Background Information

The C-C motif chemokine ligand 22 (CCL22), also known as MDC, belongs to the group of chemokines, that are both constitutively expressed under homeostatic conditions and inducible upon inflammation. CCL22 is a secreted protein that exerts chemotactic activity for monocytes, dendritic cells, natural killer cells, and for chronically activated T lymphocytes. CCL22 is induced by LPS, IL-4, and IL-13 and in T cells by TCR stimulation.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Ccl22, DCBCK, MDC, Scya22

Publications

SpeciesSample TypeTitle
mousecell culture supernatants

Int Immunopharmacol

CCL17 acts as an antitumor chemokine in micromilieu-driven immune skewing

Authors - Yadan Li