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  • Featured Product
  • KD/KO Validated

GLYR1 Polyclonal antibody

GLYR1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 14833-1-AP

Synonyms

BM045, Glyoxylate reductase 1 homolog, GLYR1, HIBDL, N PAC, N-PAC, Nuclear protein NP60, Nuclear protein of 60 kDa, Putative oxidoreductase GLYR1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GLYR1 Polyclonal antibody (14833-1-AP)

    WB analysis using 14833-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14833-1-AP (GLYR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using GLYR1 Polyclonal antibody (14833-1-AP)

    WB analysis of HL-60 using 14833-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 14833-1-AP (GLYR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GLYR1 Polyclonal antibody (14833-1-AP)

    WB analysis of HeLa using 14833-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14833-1-AP (GLYR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using GLYR1 Polyclonal antibody (14833-1-AP)

    WB analysis of Jurkat using 14833-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14833-1-AP (GLYR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using GLYR1 Polyclonal antibody (14833-1-AP)

    IP experiment of K-562 using 14833-1-AP

    IP result of anti-GLYR1 (IP:14833-1-AP, 4ug; Detection:14833-1-AP 1:500) with K-562 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GLYR1 Polyclonal antibody (14833-1-AP)

    IHC staining of mouse testis using 14833-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14833-1-AP (GLYR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GLYR1 Polyclonal antibody (14833-1-AP)

    IHC staining of mouse testis using 14833-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14833-1-AP (GLYR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GLYR1 Polyclonal antibody (14833-1-AP)

    IHC staining of human liver cancer using 14833-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14833-1-AP (GLYR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GLYR1 Polyclonal antibody (14833-1-AP)

    IHC staining of human liver cancer using 14833-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14833-1-AP (GLYR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using GLYR1 Polyclonal antibody (14833-1-AP)

    IHC staining of human colon using 14833-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 14833-1-AP (GLYR1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using GLYR1 Polyclonal antibody (14833-1-AP)

    IHC staining of human colon using 14833-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 14833-1-AP (GLYR1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GLYR1 Polyclonal antibody (14833-1-AP)

    IF Staining of HeLa using 14833-1-AP

    Immunofluorescent analysis of Hela cells, using N-PAC antibody 14833-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, Jurkat cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse testis tissue, human liver cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14833-1-AP targets GLYR1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLYR1 fusion protein Ag6637
Full Name cytokine-like nuclear factor n-pac
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC064940
Gene Symbol N-PAC
Gene ID (NCBI) 84656
RRIDAB_10859775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GLYR1 antibody 14833-1-APDownload protocol
IHC protocol for GLYR1 antibody 14833-1-APDownload protocol
IF protocol for GLYR1 antibody 14833-1-APDownload protocol
IP protocol for GLYR1 antibody 14833-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Cell

Transcription factor protein interactomes reveal genetic determinants in heart disease.

Authors - Barbara Gonzalez-Teran
View Article
mouseChIP, WB

Genomics Proteomics Bioinformatics

Npac Is a Co-factor of Histone H3K36me3 and Regulates Transcriptional Elongation in Mouse Embryonic Stem Cells.

Authors - Sue Yu
View Article
humanWB,IHC,CoIP,IF

J Exp Clin Cancer Res

Downregulation of GLYR1 contributes to microsatellite instability colorectal cancer by targeting p21 via the p38MAPK and PI3K/AKT pathways.

Authors - Zhiyan Hu
  • KD Validated
View Article