Napsin A Monoclonal antibody

Napsin A Monoclonal Antibody for FC, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1H7F2

Cat no : 60259-2-Ig

Synonyms

Asp 4, ASP4, Aspartyl protease 4, KAP, Kdap, NAP1, NAPA, NAPSA, Napsin 1, Napsin A, napsin A aspartic peptidase, Pronapsin A, SNAPA, TA01/TA02



Tested Applications

Positive IHC detected inhuman lung cancer tissue, human kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells
Positive FC detected inA549 cells, HUVEC cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:10000-1:60000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

60259-2-Ig targets Napsin A in IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Napsin A fusion protein Ag9721
Full Name napsin A aspartic peptidase
Calculated Molecular Weight 420 aa, 45 kDa
GenBank Accession NumberBC017842
Gene Symbol NAPSA
Gene ID (NCBI) 9476
RRIDAB_2881381
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).

Protocols

Product Specific Protocols
IHC protocol for Napsin A antibody 60259-2-IgDownload protocol
IF protocol for Napsin A antibody 60259-2-IgDownload protocol
FC protocol for Napsin A antibody 60259-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Commun

Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing γδ T cells.

Authors - Yanyun Du