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NARFL-Specific Polyclonal antibody

NARFL-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20209-1-AP

Synonyms

CIAO3, HPRN, IOP1, LET1L, NARFL, NARFL-Specific, PRN, Protein related to Narf

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NARFL-Specific Polyclonal antibody (20209-1-AP)

    WB analysis using 20209-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NARFL-Specific Polyclonal antibody (20209-1-AP)

    WB analysis of HEK-293 using 20209-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NARFL-Specific Polyclonal antibody (20209-1-AP)

    WB analysis of HEK-293 using 20209-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NARFL-Specific Polyclonal antibody (20209-1-AP)

    IHC staining of human kidney using 20209-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NARFL-Specific Polyclonal antibody (20209-1-AP)

    IHC staining of human kidney using 20209-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NARFL-Specific Polyclonal antibody (20209-1-AP)

    IHC staining of human heart using 20209-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NARFL-Specific Polyclonal antibody (20209-1-AP)

    IHC staining of human heart using 20209-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20209-1-AP targets NARFL-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nuclear prelamin A recognition factor-like
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 26-28 kDa
GenBank Accession NumberNM_022493
Gene Symbol NARFL
Gene ID (NCBI) 64428
RRIDAB_10694435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NARFL, also named as PRN and IOP1, belongs to the NARF family. NAFL is a component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. It is required for maturation of extramitochondrial Fe/S proteins. It seems to negatively regulate the level of HIF1A expression, although this effect could be indirect. [18270200], [16956324] The antibody is specific to NARFL.

Protocols

Product Specific Protocols
WB protocol for NARFL-Specific antibody 20209-1-APDownload protocol
IHC protocol for NARFL-Specific antibody 20209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Free Radic Biol Med

The iron-sulfur cluster assembly network component NARFL Is a key element in the cellular defense against oxidative stress.

Authors - Monique Corbin
View Article