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NAT1 Polyclonal antibody

NAT1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19188-1-AP

Synonyms

AAC1, Arylamide acetylase 1, MNAT, N acetyltransferase type 1, NAT 1, NAT1, NATI

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using NAT1 Polyclonal antibody (19188-1-AP)

    WB analysis of PC-3 using 19188-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19188-1-AP (NAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using NAT1 Polyclonal antibody (19188-1-AP)

    WB analysis of PC-3 using 19188-1-AP

    WB result of NAT1 antibody (19188-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT1 transfected PC-3 cells.

  • Western Blot (WB) analysis of human placenta tissue using NAT1 Polyclonal antibody (19188-1-AP)

    WB analysis of human placenta using 19188-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19188-1-AP (NAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using NAT1 Polyclonal antibody (19188-1-AP)

    WB analysis of A375 using 19188-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 19188-1-AP (NAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NAT1 Polyclonal antibody (19188-1-AP)

    IHC staining of human breast cancer using 19188-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 19188-1-AP (NAT1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NAT1 Polyclonal antibody (19188-1-AP)

    IHC staining of human breast cancer using 19188-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 19188-1-AP (NAT1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NAT1 Polyclonal antibody (19188-1-AP)

    IHC staining of human breast cancer using 19188-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 19188-1-AP (NAT1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NAT1 Polyclonal antibody (19188-1-AP)

    IHC staining of human breast cancer using 19188-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 19188-1-AP (NAT1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NAT1 Polyclonal antibody (19188-1-AP)

    IF Staining of HeLa using 19188-1-AP

    Immunofluorescent analysis of Hela cells, using NAT1 antibody 19188-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NAT1 Polyclonal antibody (19188-1-AP)

    IF Staining of HepG2 using 19188-1-AP

    Immunofluorescent analysis of HepG2 cells, using NAT1 antibody 19188-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inPC-3 cells, human placenta tissue, A375 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19188-1-AP targets NAT1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAT1 fusion protein Ag6280
Full Name N-acetyltransferase 1 (arylamine N-acetyltransferase)
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 32-34 kDa
GenBank Accession NumberBC047666
Gene Symbol NAT1
Gene ID (NCBI) 9
RRIDAB_10641045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NAT1 antibody 19188-1-APDownload protocol
IHC protocol for NAT1 antibody 19188-1-APDownload protocol
IF protocol for NAT1 antibody 19188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Prognostic and Clinicopathological Significance of X-Box-Binding Protein 1 and N-Acetyltransferase 1 in Gallbladder Cancer.

Authors - Rushi Liu
View Article
humanWB,CoIP

Nucleic Acids Res

Integrated multi-omics approach revealed cellular senescence landscape

Authors - Qiao Song
View Article