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NAT5 Polyclonal antibody

NAT5 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 15807-1-AP

Synonyms

N acetyltransferase 5, NAA20, NAT3, NAT5, NatB complex subunit NAT5

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NAT5 Polyclonal antibody (15807-1-AP)

    WB analysis of HeLa using 15807-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15807-1-AP (NAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NAT5 Polyclonal antibody (15807-1-AP)

    WB analysis of human brain using 15807-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15807-1-AP (NAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using NAT5 Polyclonal antibody (15807-1-AP)

    WB analysis of HL-60 using 15807-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 15807-1-AP (NAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HL-60 cells using NAT5 Polyclonal antibody (15807-1-AP)

    IP experiment of HL-60 using 15807-1-AP

    IP result of anti-NAT5 (IP:15807-1-AP, 4ug; Detection:15807-1-AP 1:500) with HL-60 cells lysate 3040ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NAT5 Polyclonal antibody (15807-1-AP)

    IF Staining of HeLa using 15807-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15807-1-AP (NAT5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, human brain tissue
Positive IP detected inHL-60 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15807-1-AP targets NAT5 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAT5 fusion protein Ag8541
Full Name N-acetyltransferase 5 (GCN5-related, putative)
Calculated Molecular Weight 178 aa, 20 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC005181
Gene Symbol NAT5
Gene ID (NCBI) 51126
RRIDAB_1640056
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NAT5 antibody 15807-1-APDownload protocol
IF protocol for NAT5 antibody 15807-1-APDownload protocol
IP protocol for NAT5 antibody 15807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

NatB Catalytic Subunit Depletion Disrupts DNA Replication Initiation Leading to Senescence in MEFs

Authors - Jasmin Elurbide
View Article
humanWB

Oncotarget

NatB-mediated protein N-α-terminal acetylation is a potential therapeutic target in hepatocellular carcinoma.

Authors - Leire Neri
View Article