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  • Featured Product
  • KD/KO Validated

NCAPH Monoclonal antibody

NCAPH Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3D2F11

Cat no : 67655-1-Ig

Synonyms

Barren homolog protein 1, BRRN, BRRN1, CAP H, CAPH, Condensin complex subunit 2, HCAP H, KIAA0074, NCAPH, XCAP H homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCAPH Monoclonal antibody (67655-1-Ig)

    WB analysis using 67655-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67655-1-Ig (NCAPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of HEK-293 cells using NCAPH Monoclonal antibody (67655-1-Ig)

    WB analysis of HEK-293 using 67655-1-Ig

    WB result of NCAPH antibody (67655-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAPH transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using NCAPH Monoclonal antibody (67655-1-Ig)

    WB analysis using 67655-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67655-1-Ig (NCAPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NCAPH Monoclonal antibody (67655-1-Ig)

    IHC staining of human liver cancer using 67655-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67655-1-Ig (NCAPH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NCAPH Monoclonal antibody (67655-1-Ig)

    IHC staining of human colon cancer using 67655-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67655-1-Ig (NCAPH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using NCAPH Monoclonal antibody (67655-1-Ig)

    IF Staining of human liver cancer using 67655-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using NCAPH antibody (67655-1-Ig, Clone: 3D2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67655-1-Ig targets NCAPH in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NCAPH fusion protein Ag27748
Full Name non-SMC condensin I complex, subunit H
Calculated Molecular Weight 741 aa, 83 kDa
Observed Molecular Weight 83-100 kDa
GenBank Accession NumberBC024211
Gene Symbol NCAPH
Gene ID (NCBI) 23397
RRIDAB_2918495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Non-SMC condensin I complex subunit H (NCAPH) is one of the three non-SMC subunits in condensin I, which belongs to a recently defined superfamily of proteins termed kleisins. Another two non-SMC subunits, CAP-D2 and CAP-G, share a highly degenerate repeating motif known as HEAT repeat. Some studies show that each subunit is essential for viability and plays an important role in mitotic chromosome architecture and segregation. In recent years, researchers found that the high expression of NCAPH was associated with poor prognosis in patients with non-small cell lung cancer and prostate cancer. Downregulation of NCAPH inhibited the proliferation, migration, and invasion of several cancer cells significantly. Moreover, NCAPH was involved in the regulation of mature chromosome condensation and DNA damage. These data suggest that NCAPH may be a key carcinogen involved in the development and progression of human malignant tumors. ( PMID: 28300828, PMID: 33311486 )

Protocols

Product Specific Protocols
WB protocol for NCAPH antibody 67655-1-IgDownload protocol
IHC protocol for NCAPH antibody 67655-1-IgDownload protocol
IF protocol for NCAPH antibody 67655-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols