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NOXA2/p67phox Monoclonal antibody

NOXA2/p67phox Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D11A3

Cat no : 67594-1-Ig

Synonyms

FLJ93058, NADPH oxidase activator 2, NCF 2, NCF2, Neutrophil cytosol factor 2, neutrophil cytosolic factor 2, NOXA2, P67 PHOX, P67PHOX

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using NOXA2/p67phox Monoclonal antibody (67594-1-Ig)

    WB analysis of HepG2 using 67594-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67594-1-Ig (NOXA2/p67phox antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using NOXA2/p67phox Monoclonal antibody (67594-1-Ig)

    WB analysis of L02 using 67594-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 67594-1-Ig (NOXA2/p67phox antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using NOXA2/p67phox Monoclonal antibody (67594-1-Ig)

    WB analysis of pig liver using 67594-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67594-1-Ig (NOXA2/p67phox antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using NOXA2/p67phox Monoclonal antibody (67594-1-Ig)

    IHC staining of human tonsillitis using 67594-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67594-1-Ig (NOXA2/p67phox antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NOXA2/p67phox Monoclonal antibody (67594-1-Ig)

    IF Staining of A431 using 67594-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using NOXA2/p67phox antibody (67594-1-Ig, Clone: 1D11A3 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, pig liver tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67594-1-Ig targets NOXA2/p67phox in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NOXA2/p67phox fusion protein Ag8691
Full Name neutrophil cytosolic factor 2
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC001606
Gene Symbol NCF2
Gene ID (NCBI) 4688
RRIDAB_2882801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NOXA2/p67phox antibody 67594-1-IgDownload protocol
IHC protocol for NOXA2/p67phox antibody 67594-1-IgDownload protocol
IF protocol for NOXA2/p67phox antibody 67594-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols