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NCLN Polyclonal antibody

NCLN Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10985-1-AP

Synonyms

NCLN, Nicalin, nicalin homolog (zebrafish), Nicastrin like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using NCLN Polyclonal antibody (10985-1-AP)

    WB analysis of mouse pancreas using 10985-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10985-1-AP (NCLN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NCLN Polyclonal antibody (10985-1-AP)

    IHC staining of human stomach cancer using 10985-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10985-1-AP (NCLN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NCLN Polyclonal antibody (10985-1-AP)

    IHC staining of human stomach cancer using 10985-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10985-1-AP (NCLN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NCLN Polyclonal antibody (10985-1-AP)

    IHC staining of human pancreas cancer using 10985-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10985-1-AP (NCLN antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NCLN Polyclonal antibody (10985-1-AP)

    IHC staining of human pancreas cancer using 10985-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10985-1-AP (NCLN antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NCLN Polyclonal antibody (10985-1-AP)

    IHC staining of human pancreas cancer using 10985-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10985-1-AP (NCLN antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NCLN Polyclonal antibody (10985-1-AP)

    IHC staining of human pancreas cancer using 10985-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10985-1-AP (NCLN antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman stomach cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10985-1-AP targets NCLN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCLN fusion protein Ag1430
Full Name nicalin homolog (zebrafish)
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60-64 kDa
GenBank Accession NumberBC013283
Gene Symbol NCLN
Gene ID (NCBI) 56926
RRIDAB_2150898
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nicalin, also named as NCLN, is a key component of a protein complex that antagonizes Nodal signaling, which in vertebrates is involved in induction of the mesoderm and endoderm. Nicalin is highest expression in pancreas and skeletal muscle, with somewhat lower expression in heart and very low expression in all other tissues examined. Endogenous nicalin has an apparent molecular mass of 60 kDa. Nicalin was also detected in a 500- to 550-kDa complex containing NOMO2.

Protocols

Product Specific Protocols
WB protocol for NCLN antibody 10985-1-APDownload protocol
IHC protocol for NCLN antibody 10985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols