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NDP Polyclonal antibody

NDP Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 15380-1-AP

Synonyms

EVR2, FEVR, ND, NDP, Norrie disease (pseudoglioma), Norrie disease protein, Norrin

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using NDP Polyclonal antibody (15380-1-AP)

    WB analysis of mouse testis using 15380-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15380-1-AP (NDP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using NDP Polyclonal antibody (15380-1-AP)

    WB analysis of mouse eye using 15380-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 15380-1-AP (NDP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse testis tissue, mouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 15380-1-AP is NDP Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDP fusion protein Ag4066
Full Name Norrie disease (pseudoglioma)
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC029901
Gene Symbol NDP
Gene ID (NCBI) 4693
RRIDAB_2878133
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDP, also named as EVR2, ND and Norrin, is a secreted regulatory protein that remains tightly associated with the extracellular matrix. Mutations in the NDP gene are associated with the Norrie disease. Signaling induced by the protein NDP regulates vascular development of vertebrate retina and controls important blood vessels in the ear. It binds with high affinity to Frizzled 4, and Frizzled 4 knockout mice exhibit abnormal vascular development of the retina. This antibody detects 11-15 kDa (monomer) and 48 kDa (polymers, refer: 25692504).

Protocols

Product Specific Protocols
WB protocol for NDP antibody 15380-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols