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NDUFB9 Polyclonal antibody

NDUFB9 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15572-1-AP

Synonyms

B22, CI B22, Complex I B22, LYR motif containing protein 3, LYRM3, NDUFB9, UQOR22

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFB9 Polyclonal antibody (15572-1-AP)

    WB analysis using 15572-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15572-1-AP (NDUFB9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFB9 Polyclonal antibody (15572-1-AP)

    IHC staining of human liver using 15572-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15572-1-AP (NDUFB9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFB9 Polyclonal antibody (15572-1-AP)

    IHC staining of human liver using 15572-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15572-1-AP (NDUFB9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFB9 Polyclonal antibody (15572-1-AP)

    IHC staining of human kidney using 15572-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15572-1-AP (NDUFB9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse pancreas tissue, rat pancreas tissue
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15572-1-AP targets NDUFB9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFB9 fusion protein Ag7916
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC007672
Gene Symbol NDUFB9
Gene ID (NCBI) 4715
RRIDAB_2267110
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NDUFB9 antibody 15572-1-APDownload protocol
IHC protocol for NDUFB9 antibody 15572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

ZHX2 emerges as a negative regulator of mitochondrial oxidative phosphorylation during acute liver injury

Authors - Yankun Zhang
View Article