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NEIL1 Monoclonal antibody

NEIL1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C6D6

Cat no : 67012-1-Ig

Synonyms

DNA glycosylase/AP lyase Neil1, Endonuclease 8 like 1, Endonuclease VIII like 1, FLJ22402, FPG1, hFPG1, NEH1, Nei homolog 1, Nei like protein 1, NEI1, NEIL1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NEIL1 Monoclonal antibody (67012-1-Ig)

    WB analysis of HeLa using 67012-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67012-1-Ig (NEIL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using NEIL1 Monoclonal antibody (67012-1-Ig)

    WB analysis of A375 using 67012-1-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 67012-1-Ig (NEIL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using NEIL1 Monoclonal antibody (67012-1-Ig)

    WB analysis of COLO 320 using 67012-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67012-1-Ig (NEIL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using NEIL1 Monoclonal antibody (67012-1-Ig)

    IHC staining of mouse spleen using 67012-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67012-1-Ig (NEIL1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using NEIL1 Monoclonal antibody (67012-1-Ig)

    IHC staining of mouse spleen using 67012-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67012-1-Ig (NEIL1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NEIL1 Monoclonal antibody (67012-1-Ig)

    IF Staining of HepG2 using 67012-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67012-1-Ig (NEIL1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, A375 cells, COLO 320 cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67012-1-Ig targets NEIL1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NEIL1 fusion protein Ag8307
Full Name nei endonuclease VIII-like 1 (E. coli)
Calculated Molecular Weight 390 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC010876
Gene Symbol NEIL1
Gene ID (NCBI) 79661
RRIDAB_2882329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEIL1, also named as NEH1 and FPG1, belongs to the FPG family. It is involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. NEIL1 acts as DNA glycosylase that recognizes and removes damaged bases. It has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. NEIL1 has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. It cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. NEIL1 has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. The increased BER activity of NEILs may represent an adaptive response against ROS-induced DNA damage resulting from aniline exposure, and could be an important mechanism for the removal of oxidative DNA lesions. (PMID:21145906)

Protocols

Product Specific Protocols
WB protocol for NEIL1 antibody 67012-1-IgDownload protocol
IHC protocol for NEIL1 antibody 67012-1-IgDownload protocol
IF protocol for NEIL1 antibody 67012-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols