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Neudesin/NENF Monoclonal antibody

Neudesin/NENF Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4G9E12

Cat no : 60131-1-Ig

Synonyms

CIR2, NENF, NEUDESIN, Neudesin/NENF, SCIRP10, SPUF, SPUF protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    WB analysis using 60131-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    WB analysis of human brain using 60131-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 60131-1-Ig (NENF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human cerebellum using 60131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human cerebellum using 60131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human heart using 60131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human heart using 60131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human colon using 60131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human colon tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human colon using 60131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human kidney using 60131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human kidney using 60131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IF Staining of HepG2 using 60131-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 60131-1-Ig(NENF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, human brain tissue, HepG2 cells, LNCaP cells, A549 cells, HCT 116 cells, Jurkat cells, pig brain tissue
Positive IHC detected inhuman cerebellum tissue, human colon tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60131-1-Ig targets Neudesin/NENF in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Neudesin/NENF fusion protein Ag8387
Full Name neuron derived neurotrophic factor
Calculated Molecular Weight 172 aa, 19 kDa
Observed Molecular Weight 19 kDa, 16 kDa
GenBank Accession NumberBC008823
Gene Symbol NENF
Gene ID (NCBI) 29937
RRIDAB_2150987
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Neudesin/NENF antibody 60131-1-IgDownload protocol
IHC protocol for Neudesin/NENF antibody 60131-1-IgDownload protocol
IF protocol for Neudesin/NENF antibody 60131-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols