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GNAS Monoclonal antibody

GNAS Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1A9C10

Cat no : 66253-1-Ig

Synonyms

AHO, C20orf45, GNAS, GNAS complex locus, GNAS1, GPSA, GSA, GSP, NESP, PHP1A, PHP1B, POH, Protein ALEX

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using GNAS Monoclonal antibody (66253-1-Ig)

    WB analysis of fetal human brain using 66253-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using GNAS Monoclonal antibody (66253-1-Ig)

    WB analysis of pig brain using 66253-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using GNAS Monoclonal antibody (66253-1-Ig)

    IHC staining of human colon using 66253-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using GNAS Monoclonal antibody (66253-1-Ig)

    IHC staining of human colon using 66253-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GNAS Monoclonal antibody (66253-1-Ig)

    IF Staining of MCF-7 using 66253-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using GNAS Monoclonal antibody (66253-1-Ig)

    IF Staining of human colon cancer using 66253-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using GNAS Monoclonal antibody (66253-1-Ig)

    IF Staining of human colon cancer using 66253-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using GNAS Monoclonal antibody (66253-1-Ig)

    IF Staining of human colon cancer using 66253-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of MCF-7 cells using GNAS Monoclonal antibody (66253-1-Ig)

    FC experiment of MCF-7 using 66253-1-Ig

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human GNAS (66253-1-Ig, Clone:1A9C10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using GNAS Monoclonal antibody (66253-1-Ig)

    FC experiment of HeLa using 66253-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human GNAS (66253-1-Ig, Clone:1A9C10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected infetal human brain tissue, pig brain tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, human colon cancer tissue
Positive FC detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66253-1-Ig targets GNAS in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GNAS fusion protein Ag20530
Full Name GNAS complex locus
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC002722
Gene Symbol GNAS
Gene ID (NCBI) 2778
RRIDAB_2881641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

Protocols

Product Specific Protocols
WB protocol for GNAS antibody 66253-1-IgDownload protocol
IHC protocol for GNAS antibody 66253-1-IgDownload protocol
IF protocol for GNAS antibody 66253-1-IgDownload protocol
FC protocol for GNAS antibody 66253-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Vet Microbiol

Porcine circovirus-like virus P1 activates pancreatic secretion pathway by interacting with CHRM3 protein

Authors - Jiaping Zhu
View Article
humanIF,WB

FASEB J

GRP78 acts as a cAMP/PKA signaling modulator through the MC4R pathway in porcine embryonic development

Authors - Geun Heo
View Article