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  • Featured Product
  • KD/KO Validated

NEU3 Monoclonal antibody

NEU3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C9C5

Cat no : 67098-1-Ig

Synonyms

cDNA FLJ78026, FLJ12388, NEU3, NEU3 protein, SIAL3

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using NEU3 Monoclonal antibody (67098-1-Ig)

    WB analysis of PC-3 using 67098-1-Ig

    WB result of NEU3 antibody (67098-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEU3 transfected PC-3 cells.

  • Western Blot (WB) analysis of various lysates using NEU3 Monoclonal antibody (67098-1-Ig)

    WB analysis using 67098-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using NEU3 Monoclonal antibody (67098-1-Ig)

    WB analysis of THP-1 using 67098-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using NEU3 Monoclonal antibody (67098-1-Ig)

    WB analysis of K-562 using 67098-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using NEU3 Monoclonal antibody (67098-1-Ig)

    WB analysis of PC-3 using 67098-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using NEU3 Monoclonal antibody (67098-1-Ig)

    WB analysis of COLO 320 using 67098-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-29 cells using NEU3 Monoclonal antibody (67098-1-Ig)

    WB analysis of HT-29 using 67098-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using NEU3 Monoclonal antibody (67098-1-Ig)

    WB analysis of K-562 using 67098-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NEU3 Monoclonal antibody (67098-1-Ig)

    IHC staining of human pancreas cancer using 67098-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67098-1-Ig (NEU3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NEU3 Monoclonal antibody (67098-1-Ig)

    IHC staining of human pancreas cancer using 67098-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67098-1-Ig (NEU3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NEU3 Monoclonal antibody (67098-1-Ig)

    IHC staining of human pancreas cancer using 67098-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67098-1-Ig (NEU3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inPC-3 cells, COLO 320 cells, HT-29 cells, K-562 cells, THP-1 cells, Caco-2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67098-1-Ig targets NEU3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NEU3 fusion protein Ag27272
Full Name sialidase 3 (membrane sialidase)
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48-52 kDa
GenBank Accession NumberNM_006656.5
Gene Symbol NEU3
Gene ID (NCBI) 10825
RRIDAB_2882403
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NEU3 antibody 67098-1-IgDownload protocol
IHC protocol for NEU3 antibody 67098-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols