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Neurofascin Polyclonal antibody

Neurofascin Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26351-1-AP

Synonyms

KIAA0756, Neurofascin, neurofascin homolog (chicken), NFASC, NRCAML

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of rat brain tissue using Neurofascin Polyclonal antibody (26351-1-AP)

    WB analysis of rat brain using 26351-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 26351-1-AP (Neurofascin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using Neurofascin Polyclonal antibody (26351-1-AP)

    IHC staining of human brain using 26351-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26351-1-AP (Neurofascin antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using Neurofascin Polyclonal antibody (26351-1-AP)

    IHC staining of human brain using 26351-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26351-1-AP (Neurofascin antibody at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inrat brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 26351-1-AP is Neurofascin Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Neurofascin fusion protein Ag24165
Full Name neurofascin homolog (chicken)
Calculated Molecular Weight 1347 aa, 150 kDa
Observed Molecular Weight 186 kDa
GenBank Accession NumberBC137013
Gene Symbol NFASC
Gene ID (NCBI) 23114
RRIDAB_2880486
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Neurofascin antibody 26351-1-APDownload protocol
IHC protocol for Neurofascin antibody 26351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Pharmacol Res

The role of circTmeff-1 in incubation of context-induced morphine craving.

Authors - Hailei Yu
View Article
humanWB

Aging (Albany NY)

Tumor-suppressive miR-3650 inhibits tumor metastasis by directly targeting NFASC in hepatocellular carcinoma.

Authors - Jian Wu
View Article
ratWB

J Cancer Prev

Gene Expression Changes by Diallyl Trisulfide Administration in Chemically-induced Mammary Tumors in Rats.

Authors - Eun-Ryeong Hahm
View Article