NFATC2 Monoclonal antibody
NFATC2 Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse
Applications
WB, IHC, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1F3B2
Cat no : 66917-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Ramos cells, Daudi cells, Jurkat cells |
| Positive IHC detected in | human breast cancer tissue, human lymphoma tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66917-1-Ig targets NFATC2 in WB, IHC, FC, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NFATC2 fusion protein Ag17990 |
| Full Name | nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 |
| Calculated Molecular Weight | 925 aa, 100 kDa |
| Observed Molecular Weight | 135-140 kDa |
| GenBank Accession Number | BC136418 |
| Gene Symbol | NFATC2 |
| Gene ID (NCBI) | 4773 |
| RRID | AB_2882244 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2), also named NFAT1, or NFATP, is a 925 amino acid protein, which is expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. NFATC2 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. NFATC2 promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. The calculated molecular weight of NFATC2 is about 97-100 kDa, but the modified NFATC2 protein is about 135 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NFATC2 antibody 66917-1-Ig | Download protocol |
| IHC protocol for NFATC2 antibody 66917-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
