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NFE2L1 Polyclonal antibody

NFE2L1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17062-1-AP

Synonyms

HBZ17, LCR F1, Locus control region factor 1, NF E2 related factor 1, NFE2 related factor 1, NFE2L1, NRF1, TCF 11, TCF11, Transcription factor 11, Transcription factor HBZ17, Transcription factor LCR F1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NFE2L1 Polyclonal antibody (17062-1-AP)

    WB analysis of HEK-293 using 17062-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17062-1-AP (NFE2L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using NFE2L1 Polyclonal antibody (17062-1-AP)

    WB analysis of NIH/3T3 using 17062-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17062-1-AP (NFE2L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NFE2L1 Polyclonal antibody (17062-1-AP)

    IF Staining of HepG2 using 17062-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFE2L1 antibody (17062-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17062-1-AP targets NFE2L1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFE2L1 fusion protein Ag10832
Full Name nuclear factor (erythroid-derived 2)-like 1
Calculated Molecular Weight 772 aa, 85 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC010623
Gene Symbol NFE2L1
Gene ID (NCBI) 4779
RRIDAB_2878342
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFE2L1, also named as HBZ17, NRF1, TCF11 and LCR-F1, belongs to the bZIP family and CNC subfamily. It activates erythroid-specific, globin gene expression. NFE2L1 is involved in mediating ascorbic acid-induced osterix expression and osteoblast differentiation via binding to the antioxidant response element of the osterix promoter. NFE2L1, produced by osteoblasts, is involved in regulating osterix expression, osteoblast differentiation, and bone formation. (PMID:19887580). This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human ESRRG.

Protocols

Product Specific Protocols
WB protocol for NFE2L1 antibody 17062-1-APDownload protocol
IF protocol for NFE2L1 antibody 17062-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Overexpression of NRF1-742 or NRF1-772 Reduces Arsenic-Induced Cytotoxicity and Apoptosis in Human HaCaT Keratinocytes.

Authors - Shuai Wang
View Article
ratWB,IHC

Stem Cell Rev Rep

EIF4A3-Induced Exosomal circLRRC8A Alleviates Granulosa Cells Senescence Via the miR-125a-3p/NFE2L1 axis

Authors - Jie Xing
View Article