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NFIL3 Polyclonal antibody

NFIL3 Polyclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC (Intra), chIP, ELISA

Conjugate

Unconjugated

Cat no : 11773-1-AP

Synonyms

E4 promoter binding protein 4, E4BP4, IL3BP1, NF IL3A, NFIL3, NFIL3A



Tested Applications

Positive WB detected inHepG2 cells, K562 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse stomach tissue, human skin cancer tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inNK92, NK92 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11773-1-AP targets NFIL3 in WB, IP, IHC, IF, FC (Intra), chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFIL3 fusion protein Ag2380
Full Name nuclear factor, interleukin 3 regulated
Calculated Molecular Weight 462 aa, 51 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC008197
Gene Symbol NFIL3
Gene ID (NCBI) 4783
RRIDAB_2267287
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFIL3, also named as E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227). The calculated molecular weight of NFIL3 is 51 kDa, but the modified NFIL3 is about 55-60 kDa.

Protocols

Product Specific Protocols
WB protocol for NFIL3 antibody 11773-1-APDownload protocol
IHC protocol for NFIL3 antibody 11773-1-APDownload protocol
IF protocol for NFIL3 antibody 11773-1-APDownload protocol
IP protocol for NFIL3 antibody 11773-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Brain

Mislocated FUS is sufficient for gain-of-toxic-function amyotrophic lateral sclerosis phenotypes in mice.

Authors - Gen Shiihashi
mouse

Theranostics

The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition.

Authors - Fangjun Yu
ratWB

Neuropharmacology

Retigabine ameliorates acute stress-induced impairment of spatial memory retrieval through regulating USP2 signaling pathways in hippocampal CA1 area.

Authors - Cai Li
mouseWB

Biochem Pharmacol

Tangeretin prevents cognitive deficit in delirium through activating RORα/γ-E4BP4 axis in mice

Authors - Min Chen
humanWB, ChIP

Front Med (Lausanne)

NFIL3 Facilitates Neutrophil Autophagy, Neutrophil Extracellular Trap Formation and Inflammation During Gout via REDD1-Dependent mTOR Inactivation.

Authors - Honghu Tang
mouseWB,chIP

Drug Metab Dispos

The Molecular Mechanism Regulating Diurnal Rhythm of Flavin-Containing Monooxygenase 5 in Mouse Liver.

Authors - Min Chen
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