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NFIL3 Monoclonal antibody

NFIL3 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2F4B4

Cat no : 66368-1-Ig

Synonyms

E4 promoter binding protein 4, E4BP4, IL3BP1, NF IL3A, NFIL3, NFIL3A

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using NFIL3 Monoclonal antibody (66368-1-Ig)

    WB analysis of human skeletal muscle using 66368-1-Ig

    Western blot analysis of NFIL3 in various cell lines and tissues using Proteintech antibody 66368-1-Ig at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of NK92 using NFIL3 Monoclonal antibody (66368-1-Ig)

    FC experiment of NK92 using 66368-1-Ig

    1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human NFIL3 (66368-1-Ig, Clone:2F4B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inhuman skeletal muscle tissue
Positive FC detected inNK92, NK92 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66368-1-Ig targets NFIL3 in WB, FC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NFIL3 fusion protein Ag2380
Full Name nuclear factor, interleukin 3 regulated
Calculated Molecular Weight 462 aa, 51 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC008197
Gene Symbol NFIL3
Gene ID (NCBI) 4783
RRIDAB_2881748
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFIL3, also named E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227).

Protocols

Product Specific Protocols
WB protocol for NFIL3 antibody 66368-1-IgDownload protocol
FC protocol for NFIL3 antibody 66368-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols