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NFKBIZ Polyclonal antibody

NFKBIZ Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 14014-1-AP

Synonyms

I kappa B zeta, IkappaB zeta, IkappaBzeta, IkB zeta, IKBZ, INAP, IκBζ, MAIL, NF kappa B inhibitor zeta, NFKBIZ



Tested Applications

Positive WB detected inHeLa cells, THP-1 cells, U-251 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14014-1-AP targets NFKBIZ in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFKBIZ fusion protein Ag5120
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 78 kDa, 64 kDa
GenBank Accession NumberBC060800
Gene Symbol NFKBIZ
Gene ID (NCBI) 64332
RRIDAB_2878000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFKBIZ, also named as NF-kappa-B inhibitor zeta, is a 718 amino acid protein, which contains 7 ANK repeats and is expressed at high levels in peripheral blood leukocytes and lung, at moderate levels in liver, placenta, and at low levels in spleen, kidney, skeletal muscle and heart. NFKBIZ exists as three isoforms and molecular weight of isoform three is a 64 kDa. The isoform one is 78 kDa protein and isoform two is a 68 kDa protein. NFKBIZ is involved in regulation of NF-kappa-B transcription factor complexes. It Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation.

Protocols

Product Specific Protocols
WB protocol for NFKBIZ antibody 14014-1-APDownload protocol
IF protocol for NFKBIZ antibody 14014-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Immunol

Single-Cell Transcriptome Analysis of Chronic Antibody-Mediated Rejection After Renal Transplantation.

Authors - Fanhua Kong
humanWB

Front Mol Neurosci

Silencing NUDT21 Attenuates the Mesenchymal Identity of Glioblastoma Cells via the NF-κB Pathway.

Authors - Jia-Cheng Lou
mouseWB

Toxicol Appl Pharmacol

Tetrachloro-p-benzoquinone induces hepatic oxidative damage and inflammatory response, but not apoptosis in mouse: The prevention of curcumin.

Authors - Demei Xu

Int J Biol Macromol

Charaterization and immunomodulatory activities of polysaccharide isolated from Pleurotus eryngii.

Authors - Duoduo Xu
ratWB

J Surg Res

PMID: 25103639

Authors - Yu Zhang
mouseIF

JCI Insight

The negative feedback loop of NF-κB/miR-376b/NFKBIZ in septic acute kidney injury.

Authors - Zhiwen Liu
  • KD Validated