NFS1 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 15370-1-AP

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Freight/Packing

Con: 59 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
A549 cells, HeLa cells, HepG2 cells, human heart tissue

Positive IP detected in:
HepG2 cells

Positive IHC detected in:
human heart tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue

Positive IF detected in:
HepG2 cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
NFS1 nitrogen fixation 1 homolog (S. cerevisiae)

Calculated molecular weight:
50 kDa

Observed molecular weight:
50 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
NFS1

Synonyms
HUSSY 08, IscS, NFS1, NIFS
Background

NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.


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