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  • Featured Product
  • KD/KO Validated

Ninein Polyclonal antibody

Ninein Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13007-1-AP

Synonyms

GSK3B interacting protein, hNinein, KIAA1565, NIN, Ninein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ninein Polyclonal antibody (13007-1-AP)

    WB analysis using 13007-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13007-1-AP (Ninein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Ninein Polyclonal antibody (13007-1-AP)

    IHC staining of mouse brain using 13007-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13007-1-AP (Ninein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Ninein Polyclonal antibody (13007-1-AP)

    IHC staining of mouse brain using 13007-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13007-1-AP (Ninein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Ninein Polyclonal antibody (13007-1-AP)

    IF Staining of HeLa using 13007-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ninein antibody (13007-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Ninein Polyclonal antibody (13007-1-AP)

    IF Staining of MCF-7 using 13007-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Ninein antibody (13007-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13007-1-AP targets Ninein in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ninein fusion protein Ag3688
Full Name ninein (GSK3B interacting protein)
Calculated Molecular Weight 2090 aa, 243 kDa
Observed Molecular Weight 220-240 kDa
GenBank Accession NumberBC034708
Gene Symbol NIN
Gene ID (NCBI) 51199
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.

Protocols

Product Specific Protocols
WB protocol for Ninein antibody 13007-1-APDownload protocol
IHC protocol for Ninein antibody 13007-1-APDownload protocol
IF protocol for Ninein antibody 13007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Dev Cell

Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia.

Authors - Quynh P H Nguyen
  • KD Validated
View Article
mouseIF

Dev Dyn

Fused (Stk36) is a ciliary protein required for central pair assembly and motile cilia orientation in the mammalian oviduct.

Authors - Nozawa Yoko Inès YI
View Article