• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NIPBL Polyclonal antibody

NIPBL Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 18792-1-AP

Synonyms

CDLS, CDLS1, Delangin, IDN3, IDN3 B, NIPBL, Nipped B homolog (Drosophila), Nipped B like protein, SCC2 homolog

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using NIPBL Polyclonal antibody (18792-1-AP)

    WB analysis of HeLa using 18792-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NIPBL Polyclonal antibody (18792-1-AP)

    WB analysis of HeLa using 18792-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NIPBL Polyclonal antibody (18792-1-AP)

    IF Staining of HeLa using 18792-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18792-1-AP (NIPBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18792-1-AP targets NIPBL in WB, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NIPBL fusion protein Ag5663
Full Name Nipped-B homolog (Drosophila)
Calculated Molecular Weight 2804 aa, 316 kDa
Observed Molecular Weight 300 kDa
GenBank Accession NumberBC033847
Gene Symbol NIPBL
Gene ID (NCBI) 25836
RRIDAB_2151288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NIPBL, also named as Nipped-B-like protein, is a 28-4 amino acid protein, which Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains and belongs to the SCC2/Nipped-B family. NIPBL probably plays a structural role in chromatin and Involve in sister chromatid cohesion, possibly by interacting with the cohesin complex. endogenous full length NIPBL which is estimated to be about 300kDa led us to believe that the NIPBL protein was deteriorated or lost by the standard protein extraction and blotting techniques.

Protocols

Product Specific Protocols
WB protocol for NIPBL antibody 18792-1-APDownload protocol
IF protocol for NIPBL antibody 18792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseChIP

Cell

Lineage-specific 3D genome organization is assembled at multiple scales by IKAROS

Authors - Yeguang Hu
View Article
humanWB

Nat Commun

C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.

Authors - Dan Liu
View Article
mouseChIP

Nat Immunol

BRWD1 orchestrates small pre-B cell chromatin topology by converting static to dynamic cohesin

Authors - Malay Mandal
View Article