NLGN1 Monoclonal antibody

NLGN1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D1A12

Cat no : 66964-1-Ig

Synonyms

KIAA1070, neuroligin 1, NLGN1



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, rabbit brain tissue, pig brain tissue, human brain tissue, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 6 publications below

Product Information

66964-1-Ig targets NLGN1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NLGN1 fusion protein Ag6356
Full Name neuroligin 1
Calculated Molecular Weight 840 aa, 94 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC032555
Gene Symbol NLGN1
Gene ID (NCBI) 22871
RRIDAB_2882287
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NLGN1, also named KIAA1070, belongs to the type-B carboxylesterase/lipase family. Neuroligin1 (NLGN1) is a main component of the excitatory glutamatergic synapses complex and plays a role in synapse assembly and function (PMID: 34340665). NLGN1 has 2 isoforms with the MV of 92 and 96 kDa. Sometimes higher molecular weight of around 116 kDa can also be observed, which is a glycosylated mature form of NLGN1 (PMID: 28841651).

Protocols

Product Specific Protocols
WB protocol for NLGN1 antibody 66964-1-IgDownload protocol
IHC protocol for NLGN1 antibody 66964-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neuron

Longitudinal single-cell transcriptional dynamics throughout neurodegeneration in SCA1

Authors - Leon Tejwani
ratWB

Neuroscience

Activity-dependent Synaptic Recruitment of Neuroligin 1 in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Authors - Ji-Yuan Zhao
mouseWB

J Ethnopharmacol

Multimodal investigation reveals the neuroprotective mechanism of Angong Niuhuang pill for intracerebral hemorrhage: Converging bioinformatics, network pharmacology, and experimental validation

Authors - Jiawei Zhou
humanWB

Aging (Albany NY)

Metastasis-related gene signature associates with immunity and predicts prognosis accurately in patients with osteosarcoma

Authors - Sen Qin
mouseWB

Neurochem Int

Mitochondrial protein prohibitin promotes learning memory recovery in mice following intracerebral hemorrhage via CAMKII/CRMP signaling pathway

Authors - Tianlin Jiang
mouseWB

Aging (Albany NY)

Longitudinal characterization of behavioral, morphological and transcriptomic changes in a tauopathy mouse model

Authors - Qing Cao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wojciech (Verified Customer) (02-08-2023)

We used this antibody to assess protein levels in human T-ALL cells by western blot. Loaded 30 ug of protein. We got a good signal with 1:5000 dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: T cells