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NMRAL1 Polyclonal antibody

NMRAL1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15765-1-AP

Synonyms

HSCARG, NMRAL1, SDR48A1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NMRAL1 Polyclonal antibody (15765-1-AP)

    WB analysis using 15765-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15765-1-AP (NMRAL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NMRAL1 Polyclonal antibody (15765-1-AP)

    WB analysis using 15765-1-AP

    Various cells were subjected to SDS PAGE followed by western blot with 15765-1-AP (NMRAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using NMRAL1 Polyclonal antibody (15765-1-AP)

    IP experiment of Jurkat using 15765-1-AP

    IP result of anti-NMRAL1 (IP:15765-1-AP, 4ug; Detection:15765-1-AP 1:1500) with Jurkat cells lysate 1560 ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using NMRAL1 Polyclonal antibody (15765-1-AP)

    IHC staining of human liver using 15765-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15765-1-AP (NMRAL1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using NMRAL1 Polyclonal antibody (15765-1-AP)

    IHC staining of human liver using 15765-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15765-1-AP (NMRAL1 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, LNCaP cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15765-1-AP targets NMRAL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NMRAL1 fusion protein Ag8401
Full Name NmrA-like family domain containing 1
Calculated Molecular Weight 299 aa, 33 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC007364
Gene Symbol NMRAL1
Gene ID (NCBI) 57407
RRIDAB_2153154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NMRAL1 antibody 15765-1-APDownload protocol
IHC protocol for NMRAL1 antibody 15765-1-APDownload protocol
IP protocol for NMRAL1 antibody 15765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Stamp2 controls macrophage inflammation through nicotinamide adenine dinucleotide phosphate homeostasis and protects against atherosclerosis.

Authors - Henrik ten Freyhaus
View Article
ratIF

Diabetes Metab Syndr Obes

STAMP2 Attenuates Cardiac Dysfunction and Insulin Resistance in Diabetic Cardiomyopathy via NMRAL1-Mediated NF-κB Inhibition in Type 2 Diabetic Rats

Authors - Zhan Gao
View Article