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NMUR1 Polyclonal antibody

NMUR1 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 14619-1-AP

Synonyms

FM 3, FM3, G protein coupled receptor 66, GPC R, GPR66, neuromedin U receptor 1, NMUR1

Validation Data Gallery

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  • Western Blot (WB) analysis of human colon tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    WB analysis of human colon using 14619-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 14619-1-AP (NMUR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    WB analysis of mouse pancreas using 14619-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14619-1-AP (NMUR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human stomach tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    WB analysis of human stomach using 14619-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 14619-1-AP (NMUR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    IHC staining of mouse brain using 14619-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14619-1-AP (NMUR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    IHC staining of mouse brain using 14619-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14619-1-AP (NMUR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    IHC staining of human colon using 14619-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    IHC staining of human colon using 14619-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    IHC staining of human testis using 14619-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    IHC staining of human testis using 14619-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using NMUR1 Polyclonal antibody (14619-1-AP)

    FC experiment of SH-SY5Y using 14619-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug NMUR1 antibody (14619-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inhuman colon tissue, human stomach tissue, mouse pancreas tissue
Positive IHC detected inmouse brain tissue, human colon tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14619-1-AP targets NMUR1 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NMUR1 fusion protein Ag6235
Full Name neuromedin U receptor 1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC036543
Gene Symbol NMUR1
Gene ID (NCBI) 10316
RRIDAB_2267310
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuromedin-U (NMU) is a widely expressed peptide with the highest abundance in the central nervous system (CNS) and intestinal tract of nearly all vertebrate species. NMU mediates several physiological functions via its two cognate receptors, NMUR1 and NMUR2 (PMID:37102164). NMUR1(NmU receptor 1) has the highest expression in peripheral tissues, particularly the GI tract, pancreas, uterus, and testes but has also been reported in peripheral nerve terminals (PMID:10783389).

Protocols

Product Specific Protocols
WB protocol for NMUR1 antibody 14619-1-APDownload protocol
IHC protocol for NMUR1 antibody 14619-1-APDownload protocol
FC protocol for NMUR1 antibody 14619-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols