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RPH3AL Polyclonal antibody

RPH3AL Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 15297-1-AP

Synonyms

No C2 domains protein, NOC2, Rab effector Noc2, Rabphilin 3A like protein, RPH3AL

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using RPH3AL Polyclonal antibody (15297-1-AP)

    WB analysis of human liver using 15297-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15297-1-AP (RPH3AL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using RPH3AL Polyclonal antibody (15297-1-AP)

    WB analysis of L02 using 15297-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15297-1-AP (RPH3AL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using RPH3AL Polyclonal antibody (15297-1-AP)

    IP experiment of HepG2 using 15297-1-AP

    IP result of anti-RPH3AL (IP:15297-1-AP, 4ug; Detection:15297-1-AP 1:500) with HepG2 cells lysate 2400ug.

Tested Applications

Positive WB detected inhuman liver tissue, L02 cells
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15297-1-AP targets RPH3AL in WB, IP,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPH3AL fusion protein Ag7552
Full Name rabphilin 3A-like (without C2 domains)
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC005153
Gene Symbol RPH3AL
Gene ID (NCBI) 9501
RRIDAB_2181270
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rabphilin-3A-like (RPH3AL), also known as Noc2, is a Rab effector protein with a molecular weight of 38 kDa. It is expressed abundantly in endocrine cells but at low levels in exocrine tissues, playing a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells, and also having a key role in INS secretion by pancreatic cells. RPH3AL functions as a potential RAB3B effector protein in epithelial cells, and RPH3AL gene is likely a tumor suppressor.

Protocols

Product Specific Protocols
WB protocol for RPH3AL antibody 15297-1-APDownload protocol
IP protocol for RPH3AL antibody 15297-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Sci

Rab2a and Rab27a cooperatively regulate transition from granule maturation to exocytosis through the dual effector Noc2.

Authors - Kohichi Matsunaga
View Article
humanWB

Clin Chim Acta

Detection of autoantibodies against Rabphilin-3A-like protein as a potential biomarker in patient's sera of colorectal cancer.

Authors - Jinn-Shiun Chen
View Article