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  • Featured Product
  • KD/KO Validated

NOP14 Polyclonal antibody

NOP14 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26854-1-AP

Synonyms

C4orf9, NOL14, NOP14, Nucleolar complex protein 14, Nucleolar protein 14, RES4 25, RES425

Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-453s cells using NOP14 Polyclonal antibody (26854-1-AP)

    WB analysis of MDA-MB-453s using 26854-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using NOP14 Polyclonal antibody (26854-1-AP)

    WB analysis of BxPC-3 using 26854-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using NOP14 Polyclonal antibody (26854-1-AP)

    WB analysis of MCF-7 using 26854-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using NOP14 Polyclonal antibody (26854-1-AP)

    WB analysis of mouse lung using 26854-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using NOP14 Polyclonal antibody (26854-1-AP)

    WB analysis of rat kidney using 26854-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using NOP14 Polyclonal antibody (26854-1-AP)

    WB analysis of BxPC-3 using 26854-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NOP14 Polyclonal antibody (26854-1-AP)

    IF Staining of HepG2 using 26854-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 26854-1-AP (NOP14 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMDA-MB-453s cells, BxPC-3 cells, MCF-7 cells, mouse lung tissue, rat kidney tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26854-1-AP is NOP14 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOP14 fusion protein Ag25476
Full Name NOP14 nucleolar protein homolog (yeast)
Calculated Molecular Weight 98 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC026035
Gene Symbol NOP14
Gene ID (NCBI) 8602
RRIDAB_2880657
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NOP14 antibody 26854-1-APDownload protocol
IF protocol for NOP14 antibody 26854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Cell Prolif

CCND1, NOP14 and DNMT3B are involved in miR-502-5p-mediated inhibition of cell migration and proliferation in bladder cancer.

Authors - Yufan Ying
  • KD Validated
View Article
humanWB,IHC,IF

Aging (Albany NY)

Exploring Cancer Dependency Map genes and immune subtypes in colon cancer, in which TIGD1 contributes to colon cancer progression

Authors - Guoyang Zhang
View Article
humanWB

Int J Biol Macromol

CircNOP14 increases the radiosensitivity of hepatocellular carcinoma via inhibition of Ku70-dependent DNA damage repair

Authors - Letao Lin
View Article