NOP2 Polyclonal antibody

NOP2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10448-1-AP

Synonyms

NOL1, NOP120, NOP2, NSUN1, Nucleolar protein 1, Nucleolar protein 2 homolog, p120



Tested Applications

Positive WB detected inA2780 cells, C6 cells, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10448-1-AP targets NOP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOP2 fusion protein Ag0498
Full Name NOP2 nucleolar protein homolog (yeast)
Calculated Molecular Weight 120 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC000656
Gene Symbol NOP2
Gene ID (NCBI) 4839
RRIDAB_2282772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOL1, (synonyms: p120, NSUN1, NOP120), is a 120 kDa proliferating-cell nucleolar antigen and is the most cancer specific of the proliferation-associated nucleolar proteins identified thus far. NOL1 is expressed in G1 and peaks during the early S phase of the cell cycle and it has not been detected in benign tumors and most normal resting tissues. Overexpression of NOL1 caused the transformation of NIH 3T3 cells and expression of an antisense NOL1 construct inhibited the growth of NIH 3T3 cells. NOL is localized in a novel nucleolar microfibrillar structure, and contains, consecutively, four major domains: a basic domain, an acidic domain, a hydrophobic and methionine-rich domain, and a domain rich in cysteine and proline residues. The gene for human NOL1 was assigned to chromosome 12p13.

Protocols

Product Specific Protocols
WB protocol for NOP2 antibody 10448-1-APDownload protocol
IHC protocol for NOP2 antibody 10448-1-APDownload protocol
IF protocol for NOP2 antibody 10448-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nucleic Acids Res

DNA damage-induced inhibition of rRNA synthesis by DNA-PK and PARP-1.

Authors - Calkins Anne S AS
humanWB

Mol Biol Cell

RRP1B targets PP1 to mammalian cell nucleoli and is associated with Pre-60S ribosomal subunits.

Authors - Chamousset Delphine D
humanWB

Transl Cancer Res

Effect of NOP2 knockdown on colon cancer cell proliferation, migration, and invasion.

Authors - Jinling Bi
humanWB

iScience

Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges

Authors - Tamami Miyagi
mouseIF

Cell Mol Life Sci

N4-acetylcytidine of Nop2 mRNA is required for the transition of morula-to-blastocyst

Authors - Mengyun Wang
humanWB,IF,IP

Cell Rep

ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells

Authors - M Carmen Lafita-Navarro