NOP56 Polyclonal antibody

NOP56 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18181-1-AP

Synonyms

NOL5A, NOP56, Nucleolar protein 56, Nucleolar protein 5A



Tested Applications

Positive WB detected inNIH/3T3 cells, A549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

18181-1-AP targets NOP56 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOP56 fusion protein Ag12882
Full Name NOP56 ribonucleoprotein homolog (yeast)
Calculated Molecular Weight 594 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC104791
Gene Symbol NOP56
Gene ID (NCBI) 10528
RRIDAB_2152323
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Box C/D RNA-protein complexes (RNPs) guide the 2'-O-methylation of nucleotides in eukaryotic ribosomal RNAs. The box C/D and C'/D' RNP subcomplexes are each assembled with three sRNP core proteins. The Nop56 core protein mediates crucial protein-protein interactions required for both sRNP assembly and the methyltransferase reaction by bridging the L7Ae and fibrillarin core proteins [PMID:22496443]. NOP56 is a component of the box C/D small nucleolar ribonucleoprotein complexes that direct 2-prime-O-methylation of pre-ribosomal RNA (rRNA) during its maturation. It is predicted to function in an early to middle step in pre-rRNA processing [PMID:12777385].

Protocols

Product Specific Protocols
WB protocol for NOP56 antibody 18181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Sci

Discovery of a novel HDACi structure that inhibits the proliferation of ovarian cancer cells in vivo and in vitro.

Authors - Miao Bai
mouseWB

iScience

BMAL1 Associates with NOP58 in the Nucleolus and Contributes to Pre-rRNA Processing.

Authors - Marlene Cervantes