NOP58 Polyclonal antibody

NOP58 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14409-1-AP

Synonyms

HSPC120, NOL5, NOP5, NOP5/NOP58, NOP58, Nucleolar protein 5, Nucleolar protein 58



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, MCF-7 cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14409-1-AP targets NOP58 in WB, RIP, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOP58 fusion protein Ag5740
Full Name NOP58 ribonucleoprotein homolog (yeast)
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC032592
Gene Symbol NOP58
Gene ID (NCBI) 51602
RRIDAB_2152331
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The box C/D snoRNPs,is named for the short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. The box C/D sequences are also required for multiple aspects. of snoRNA biogenesis, including: RNA stability, intronic processing, nucleolar targeting, nuclear retention, and 59 trimethylguanosine cap formation [PMID:9649444,8878486]. Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins [PMID:10606270].

Protocols

Product Specific Protocols
WB protocol for NOP58 antibody 14409-1-APDownload protocol
IP protocol for NOP58 antibody 14409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Elife

The dyskerin ribonucleoprotein complex as an OCT4/SOX2 coactivator in embryonic stem cells.

Authors - Yick W Fong
humanWB,CoIP

J Virol

Protein-RNA Interactome Analysis Reveals Wide Association of Kaposi's Sarcoma-Associated Herpesvirus ORF57 with Host Noncoding RNAs and Polysomes.

Authors - Beatriz Alvarado-Hernandez
CoIP,RIP

iScience

BMAL1 Associates with NOP58 in the Nucleolus and Contributes to Pre-rRNA Processing.

Authors - Marlene Cervantes
humanWB

Dig Dis Sci

CYP1B1-AS1 Delays the Malignant Progression of Colorectal Cancer by Binding with NOP58

Authors - Zhao Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Marlene (Verified Customer) (02-25-2019)

Immunoprecipitation:From mouse liver lysate4ug of antibody per sampleIncubated overnight at 4C

  • Applications: Immunoprecipitation,
  • Cell Tissue Type: Mouse liver tissue