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NOTUM Monoclonal antibody

NOTUM Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F6D5

Cat no : 66956-1-Ig

Synonyms

NOTUM, Protein notum homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOTUM Monoclonal antibody (66956-1-Ig)

    WB analysis using 66956-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NOTUM Monoclonal antibody (66956-1-Ig)

    WB analysis of HepG2 using 66956-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using NOTUM Monoclonal antibody (66956-1-Ig)

    WB analysis of MCF-7 using 66956-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using NOTUM Monoclonal antibody (66956-1-Ig)

    WB analysis of T-47D using 66956-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using NOTUM Monoclonal antibody (66956-1-Ig)

    IHC staining of human small intestine using 66956-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66956-1-Ig (NOTUM antibody) at dilution of 1:5000 (under 10x lens)..

  • Immunohistochemistry (IHC) staining of human small intestine tissue using NOTUM Monoclonal antibody (66956-1-Ig)

    IHC staining of human small intestine using 66956-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66956-1-Ig (NOTUM antibody) at dilution of 1:5000 (under 40x lens)..

Tested Applications

Positive WB detected inMCF-7 cells, HepG2 cells, T-47D cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66956-1-Ig targets NOTUM in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NOTUM fusion protein Ag6338
Full Name notum pectinacetylesterase homolog (Drosophila)
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC060882
Gene Symbol NOTUM
Gene ID (NCBI) 147111
RRIDAB_2882279
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NOTUM antibody 66956-1-IgDownload protocol
IHC protocol for NOTUM antibody 66956-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols