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NPHP1 Polyclonal antibody

NPHP1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 25971-1-AP

Synonyms

JBTS4, Nephrocystin 1, nephronophthisis 1 (juvenile), NPHP1, SLSN1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using NPHP1 Polyclonal antibody (25971-1-AP)

    IHC staining of mouse testis using 25971-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25971-1-AP (NPHP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NPHP1 Polyclonal antibody (25971-1-AP)

    IHC staining of mouse testis using 25971-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25971-1-AP (NPHP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25971-1-AP targets NPHP1 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPHP1 fusion protein Ag22965
Full Name nephronophthisis 1 (juvenile)
Calculated Molecular Weight 83 kDa
GenBank Accession NumberBC062574
Gene Symbol NPHP1
Gene ID (NCBI) 4867
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for NPHP1 antibody 25971-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols