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NRXN1 Polyclonal antibody

NRXN1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 55051-1-AP

Synonyms

Hs.22998, KIAA0578, neurexin 1, Neurexin 1 alpha, Neurexin I alpha, NRXN1

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using NRXN1 Polyclonal antibody (55051-1-AP)

    WB analysis of SH-SY5Y using 55051-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55051-1-AP (NRXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NRXN1 Polyclonal antibody (55051-1-AP)

    IF Staining of mouse brain using 55051-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NRXN1 antibody (55051-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NRXN1 Polyclonal antibody (55051-1-AP)

    IF Staining of mouse brain using 55051-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NRXN1 antibody (55051-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55051-1-AP targets NRXN1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name neurexin 1
Calculated Molecular Weight 162 kDa
Observed Molecular Weight 160-170 kDa
GenBank Accession NumberNM_004801
Gene Symbol NRXN1
Gene ID (NCBI) 9378
RRIDAB_10859257
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRXN1, also named as KIAA0578, belongs to the neurexin family. It is a neuronal cell surface protein that may be involved in cell recognition and cell adhesion. NRXN1 may mediate intracellular signaling. The antibody is specific to NRXN1 alpha.

Protocols

Product Specific Protocols
WB protocol for NRXN1 antibody 55051-1-APDownload protocol
IF protocol for NRXN1 antibody 55051-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols