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NT5C Polyclonal antibody

NT5C Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC,ELISA

Conjugate

Unconjugated

Cat no : 16577-1-AP

Synonyms

5', 3' nucleotidase, cytosolic, cdN, Deoxy 5 nucleotidase 1, DNT, dNT 1, DNT1, NT5C, P5N2, PN I, PN II, UMPH2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using NT5C Polyclonal antibody (16577-1-AP)

    IHC staining of mouse skeletal muscle using 16577-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16577-1-AP (NT5C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using NT5C Polyclonal antibody (16577-1-AP)

    IHC staining of mouse skeletal muscle using 16577-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16577-1-AP (NT5C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using NT5C Polyclonal antibody (16577-1-AP)

    IHC staining of human pancreas using 16577-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16577-1-AP (NT5C Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using NT5C Polyclonal antibody (16577-1-AP)

    IHC staining of human pancreas using 16577-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16577-1-AP (NT5C Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inmouse skeletal muscle tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16577-1-AP targets NT5C in IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NT5C fusion protein Ag9862
Full Name 5', 3'-nucleotidase, cytosolic
Calculated Molecular Weight 201 aa, 23 kDa
GenBank Accession NumberBC017454
Gene Symbol NT5C
Gene ID (NCBI) 30833
RRIDAB_2153940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NT5C, also named as DNT1 and UMPH2, has two isoforms with calculated MW 23 kDa and 13 kDa.

Protocols

Product Specific Protocols
IHC protocol for NT5C antibody 16577-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols