TrkB Polyclonal antibody
TrkB Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig and More (1)
Applications
WB, Peptide array, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 13129-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, mouse spinal cord tissue, pig cerebellum tissue, human cerebellum tissue, human stomach tissue, rat brain tissue, mouse cerebellum tissue |
| Positive IP detected in | mouse cerebellum tissue |
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 51 publications below |
| IHC | See 5 publications below |
| IF | See 6 publications below |
| CoIP | See 1 publications below |
Product Information
13129-1-AP targets TrkB in WB, Peptide array, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TrkB fusion protein Ag3790 |
| Full Name | neurotrophic tyrosine kinase, receptor, type 2 |
| Calculated Molecular Weight | 822 aa, 92 kDa |
| Observed Molecular Weight | 90-95 kDa, 140-145 kDa |
| GenBank Accession Number | BC031835 |
| Gene Symbol | NTRK2 |
| Gene ID (NCBI) | 4915 |
| RRID | AB_2155156 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance through regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes full length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TrkB antibody 13129-1-AP | Download protocol |
| IHC protocol for TrkB antibody 13129-1-AP | Download protocol |
| IF protocol for TrkB antibody 13129-1-AP | Download protocol |
| IP protocol for TrkB antibody 13129-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Chemosphere Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny. | ||
Oxid Med Cell Longev Dl-3-n-Butylphthalide Improves Neuroinflammation in Mice with Repeated Cerebral Ischemia-Reperfusion Injury through the Nrf2-Mediated Antioxidant Response and TLR4/MyD88/NF-κB Signaling Pathway. | ||
J Affect Disord Prenatal alcohol exposure enhanced alcohol preference and susceptibility to PTSD in a sex-dependent manner through the synaptic HCN1 channel | ||
ACS Chem Neurosci Tetrahydropalmatine Regulates BDNF through TrkB/CAM Interaction to Alleviate the Neurotoxicity Induced by Methamphetamine. | ||
Am J Cancer Res LMP1 promotes nasopharyngeal carcinoma metastasis through NTRK2-mediated anoikis resistance. | ||
Cell Biosci A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation. |
