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NUBP2 Polyclonal antibody

NUBP2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15409-1-AP

Synonyms

CFD1, NBP 2, NUBP1, NUBP2, Nucleotide binding protein 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NUBP2 Polyclonal antibody (15409-1-AP)

    WB analysis using 15409-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15409-1-AP (NUBP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NUBP2 Polyclonal antibody (15409-1-AP)

    WB analysis using 15409-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15409-1-AP (NUBP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NUBP2 Polyclonal antibody (15409-1-AP)

    WB analysis using 15409-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15409-1-AP (NUBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using NUBP2 Polyclonal antibody (15409-1-AP)

    WB analysis of mouse pancreas using 15409-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15409-1-AP (NUBP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using NUBP2 Polyclonal antibody (15409-1-AP)

    IHC staining of human skeletal muscle using 15409-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15409-1-AP (NUBP2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using NUBP2 Polyclonal antibody (15409-1-AP)

    IHC staining of human skeletal muscle using 15409-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15409-1-AP (NUBP2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using NUBP2 Polyclonal antibody (15409-1-AP)

    IHC staining of human pancreas using 15409-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 15409-1-AP (NUBP2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using NUBP2 Polyclonal antibody (15409-1-AP)

    IHC staining of human pancreas using 15409-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 15409-1-AP (NUBP2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using NUBP2 Polyclonal antibody (15409-1-AP)

    IF Staining of L02 using 15409-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed L02 cells using NUBP2 antibody (15409-1-AP) at dilution of 1:200 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse pancreas tissue, L02 cells, U-251 cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman skeletal muscle tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15409-1-AP targets NUBP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUBP2 fusion protein Ag7676
Full Name nucleotide binding protein 2 (MinD homolog, E. coli)
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberBC002768
Gene Symbol NUBP2
Gene ID (NCBI) 10101
RRIDAB_10858630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NUBP2 antibody 15409-1-APDownload protocol
IHC protocol for NUBP2 antibody 15409-1-APDownload protocol
IF protocol for NUBP2 antibody 15409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Struct Mol Biol

Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators.

Authors - Nima N Naseri
View Article
humanWB

J Biol Chem

Iron-Regulated Assembly of the Cytosolic Iron-Sulfur Cluster Biogenesis Machinery.

Authors - Xiaorui Fan
View Article