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  • KD/KO Validated

MTH1 Polyclonal antibody

MTH1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16705-1-AP

Synonyms

8 oxo dGTPase, MTH 1, MTH1, Nudix motif 1, NUDT1



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, PC-3 cells
Positive IHC detected inhuman lung cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16705-1-AP targets MTH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTH1 fusion protein Ag10103
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 1
Calculated Molecular Weight179aa,20 kDa; 197aa,23 kDa
Observed Molecular Weight 18-24 kDa
GenBank Accession NumberBC014618
Gene Symbol NUDT1
Gene ID (NCBI) 4521
RRIDAB_2153822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTH1, also known as NUDT1 or 8-Oxo-dGTPase, is an important repair enzyme that prevents oxidative stress-induced DNA damage through hydrolyzing mutagenic 8-oxo-dGTP into 8-oxo-dGMP. MTH1 is expressed in postmitotic neurons as well as in proliferative tissues, and it is localized both in the mitochondria and nucleus. MTH1 might be a useful marker of oxidative stress and its level increased upon oxidative stress. Increased expression of MTH1 were also found in various cancer tissues and neurodegenerative diseases.

Protocols

Product Specific Protocols
WB protocol for MTH1 antibody 16705-1-APDownload protocol
IHC protocol for MTH1 antibody 16705-1-APDownload protocol
IF protocol for MTH1 antibody 16705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Farnesyl phenolic enantiomers as natural MTH1 inhibitors from Ganoderma sinense.

Authors - Ya Gao
  • KD Validated
mouseWB

Colloids Surf B Biointerfaces

Ultrasound-triggered reactive oxygen species effector nanoamplifier for enhanced combination therapy of mutant p53 tumors.

Authors - Xiang Wang
mouseWB

Photodiagnosis Photodyn Ther

Cepharanthine synergizes with photodynamic therapy for boosting ROS-driven DNA damage and suppressing MTH1 as a potential anti-cancer strategy

Authors - Zi-Yi Yang