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NUDT3/4/10/11 Polyclonal antibody

NUDT3/4/10/11 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10912-1-AP

Synonyms

APS1, ASP1, DIPP 3 beta, DIPP3 beta, DIPP3b, DIPP3beta, hAps1, hDIPP3beta, Nudix motif 11, NUDT11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUDT3/4/10/11 Polyclonal antibody (10912-1-AP)

    WB analysis using 10912-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10912-1-AP (NUDT11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NUDT3/4/10/11 Polyclonal antibody (10912-1-AP)

    WB analysis of human brain using 10912-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10912-1-AP (NUDT11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using NUDT3/4/10/11 Polyclonal antibody (10912-1-AP)

    WB analysis of mouse brain using 10912-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10912-1-AP (NUDT11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NUDT3/4/10/11 Polyclonal antibody (10912-1-AP)

    IHC staining of mouse testis using 10912-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NUDT3/4/10/11 Polyclonal antibody (10912-1-AP)

    IHC staining of mouse testis using 10912-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NUDT3/4/10/11 Polyclonal antibody (10912-1-AP)

    IHC staining of mouse testis using 10912-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NUDT3/4/10/11 Polyclonal antibody (10912-1-AP)

    IHC staining of human ovary tumor using 10912-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NUDT3/4/10/11 Polyclonal antibody (10912-1-AP)

    IHC staining of human ovary tumor using 10912-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NUDT3/4/10/11 Polyclonal antibody (10912-1-AP)

    IHC staining of mouse testis using 10912-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected inmouse testis tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10912-1-AP targets NUDT3/4/10/11 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUDT3/4/10/11 fusion protein Ag1346
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 11
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC009942
Gene Symbol NUDT11
Gene ID (NCBI) 55190
RRIDAB_2153836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUDT11 (nucleoside diphosphate linked moiety X-type motif 11), also named as DIPP3B or APS1, belongs to the subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (PMID: 12105228, 12689335). Five human isoforms of DIPP have been found and named as DIPP type 1(NUDT3), type 2α/β(NUDT4), type 3α(NUDT10) and type 3β(NUDT11), which have the similar molecular weight and high homology (PMID: 12105228). This antibody can recognize all the 5 isoforms of DIPP (NUDT3/4/10/11).

Protocols

Product Specific Protocols
WB protocol for NUDT3/4/10/11 antibody 10912-1-APDownload protocol
IHC protocol for NUDT3/4/10/11 antibody 10912-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols