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gp210 Polyclonal antibody

gp210 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16909-1-AP

Synonyms

gp210, KIAA0906, Nuclear pore protein gp210, nucleoporin 210kDa, Nucleoporin Nup210, NUP210, POM210

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using gp210 Polyclonal antibody (16909-1-AP)

    WB analysis of mouse lung using 16909-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16909-1-AP (gp210 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using gp210 Polyclonal antibody (16909-1-AP)

    WB analysis of MCF-7 using 16909-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16909-1-AP (gp210 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using gp210 Polyclonal antibody (16909-1-AP)

    WB analysis of mouse thymus using 16909-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16909-1-AP (gp210 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using gp210 Polyclonal antibody (16909-1-AP)

    IP experiment of HeLa using 16909-1-AP

    IP result of anti-gp210 (IP:16909-1-AP, 4ug; Detection:16909-1-AP 1:300) with HeLa cells lysate 3040 ug.

Tested Applications

Positive WB detected inmouse lung tissue, mouse thymus tissue, MCF-7 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16909-1-AP targets gp210 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen gp210 fusion protein Ag10533
Full Name nucleoporin 210kDa
Calculated Molecular Weight967aa,106 kDa; 1887aa,205 kDa
Observed Molecular Weight 205 kDa
GenBank Accession NumberBC067089
Gene Symbol NUP210
Gene ID (NCBI) 23225
RRIDAB_2157365
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for gp210 antibody 16909-1-APDownload protocol
IP protocol for gp210 antibody 16909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols