- Featured Product
- KD/KO Validated
NXF1 Polyclonal antibody
NXF1 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 10328-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293T cells, K-562 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 8 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
10328-1-AP targets NXF1 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NXF1 fusion protein Ag0413 |
| Full Name | nuclear RNA export factor 1 |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 66-70 kDa |
| GenBank Accession Number | BC004904 |
| Gene Symbol | NXF1 |
| Gene ID (NCBI) | 10482 |
| RRID | AB_2236500 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. There are some phosphorylation modification sites center on the N-terminal of NXF1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NXF1 antibody 10328-1-AP | Download protocol |
| IF protocol for NXF1 antibody 10328-1-AP | Download protocol |
| IP protocol for NXF1 antibody 10328-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol Inhibition of pyrimidine synthesis reverses viral virulence factor-mediated block of mRNA nuclear export.
| ||
Nucleic Acids Res Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy. | ||
PLoS Genet Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells.
| ||
Mol Cell Biol Fragile X mental retardation protein FMRP binds mRNAs in the nucleus.
| ||
Chromosoma XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1. |
