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NXN Monoclonal antibody

NXN Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2C6A4

Cat no : 66603-1-Ig

Synonyms

NRX, nucleoredoxin, NXN, TRG 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NXN Monoclonal antibody (66603-1-Ig)

    WB analysis using 66603-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66603-1-Ig (NXN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NXN Monoclonal antibody (66603-1-Ig)

    IHC staining of human kidney using 66603-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66603-1-Ig (NXN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NXN Monoclonal antibody (66603-1-Ig)

    IHC staining of human kidney using 66603-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66603-1-Ig (NXN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using NXN Monoclonal antibody (66603-1-Ig)

    IF Staining of HEK-293 using 66603-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NXN antibody (66603-1-Ig, Clone: 2C6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66603-1-Ig targets NXN in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NXN fusion protein Ag9248
Full Name nucleoredoxin
Calculated Molecular Weight 435 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC009327
Gene Symbol NXN
Gene ID (NCBI) 64359
RRIDAB_2881963
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.

Protocols

Product Specific Protocols
WB protocol for NXN antibody 66603-1-IgDownload protocol
IHC protocol for NXN antibody 66603-1-IgDownload protocol
IF protocol for NXN antibody 66603-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols